Analysis of RT-qPCR Data
DOI:
https://doi.org/10.6000/1929-6029.2012.01.02.10Keywords:
RT-qPCR, DNA, miRNA, Cq value, amplification efficiency, background correction, normalisation, reference gene, quality control, statistical analysisAbstract
We give a brief overview of the necessary steps in the analysis of real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR) data. We cover determination of amplification efficiency, background correction, normalization, quality control, and statistical analysis.
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Copyright (c) 2012 James R. Perkins, Nor Izayu Abdul Rahman, Matthias Kohl
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