Alpha Diversity Analysis of Microbiota Dysbiosis in Normal and Colorectal Cancer of Mice Feces
DOI:
https://doi.org/10.6000/1929-6029.2025.14.46Keywords:
Colorectal cancer, gut microbiota, Next Generation Sequencing, dysbiosis, alpha diversityAbstract
Background: Colorectal cancer development is influenced by both environmental and genetic factors, with the gut microbiota playing a significant role. This research investigates how alterations in gut microbiota are associated with the incidence, progression, prognosis, and early detection of CRC.
Methods: An experimental laboratory study was carried out using Sprague Dawley rats that were induced with azoxymethane (AOM) and Dextran Sodium Sulfate (DSS). The thirty rats were divided into three groups: normal, cancer-induced, and treatment. The fecal microbiota profiles were examined through Next Generation Sequencing (NGS), and the data were analyzed for alpha diversity, highlighting the dynamics of the microbial community.
Results: The cancer-induced group (K2 Plus) exhibited the highest microbial diversity across Shannon, Simpson, Chao1, and PD Whole Tree indices, while the treatment group (P2 Plus) demonstrated the lowest.
Conclusion: These findings suggest that the increase in diversity observed in cancer-induced mice reflects disruption of community stability and blooming of pathobionts. Conversely, treatment with Lactococcus lactis D4 reduced diversity, potentially by selectively suppressing pro-inflammatory or pathogenic taxa, indicating a beneficial probiotic effect in mitigating dysbiosis associated with colorectal cancer.
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